Specifically and Reversibly Immobilizing Proteins/Enzymes to Nitriolotriacetic??Acid??Modified Mesoporous Silicas through Histidine Tags for Purification or Catalysis

…, MR Liang, YC Lin, CT Chen

Index: Lin, Yu-Chung; Liang, Ming-Ren; Lin, Yu-Chen; Chen, Chao-Tsen Chemistry - A European Journal, 2011 , vol. 17, # 46 p. 13059 - 13067

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Citation Number: 22

Abstract

Abstract Six nitriolotriacetic-acid-modified ordered mesoporous silicas (NTA-OMPSs) with different pore sizes and surface features for specific and reversible protein immobilization were fabricated and characterized. Specific immobilization of a genetically engineered undecaprenyl pyrophosphate synthase (UPPs) from cell lysate and a chemically modified His-tagged horseradish peroxidase (HRP) in these Ni-NTA-OMPSs through histidine ...