Detailed binding sites of the antibiotics vancomycin and ristocetin A: determination of intermolecular distances in antibiotic/substrate complexes by use of the time- …

DH Williams, MP Williamson, DW Butcher…

Index: Williams; Williamson; Butcher; Hammond Journal of the American Chemical Society, 1983 , vol. 105, # 5 p. 1332 - 1339

Full Text: HTML

Citation Number: 136

Abstract

Abstract: The binding site of the antibiotic vancomycin for the peptide cell-wall analogue Ac- D-Ala-D-Ala has been studied by nuclear Overhauser effect difference spectroscopy (NOEDS). Intramolecular nuclear Overhauser effects (NOEs), observed between protons of the antibiotic in the 'H spectrum of the vancomycin/Ac-D-Ala-D-Ala complex, confirm the formation of a carboxylate binding pocket in the bound state of the antibiotic. This pocket is ...