A new carboxylesterase from Brevibacterium linens IFO 12171 responsible for the conversion of 1, 4-butanediol diacrylate to 4-hydroxybutyl acrylate: purification, …

Y Sakai, J Ishikawa, S Fukasaka…

Index: Bioscience, Biotechnology and Biochemistry, , vol. 63, # 4 p. 688 - 697

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Citation Number: 20

Abstract

... Kyoto University, Kiiashirakawa, Sakyo-ku, Kyoto 606-8502, Japan *Departmeni of Biotechnology, T otiori ... follows: mobile phase, acetonitrile, methanol, and H20 (4:1:3, vo1/ vol); flow rate ... using a fast protein liq- uid chromatography system (Amersham Pharmacia Biotech), on a ...