Development of a selective enzyme-linked immunosorbent assay for 1-naphthol-the major metabolite of carbaryl (1-naphthyl N-methylcarbamate)

…, MP Marco, BD Hammock

Index: Kraemer, Petra M.; Marco, Maria-Pilar; Hammock, Bruce D. Journal of Agricultural and Food Chemistry, 1994 , vol. 42, # 4 p. 934 - 943

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Citation Number: 52

Abstract

A selective enzyme-linked immunosorbent assay for l-naphthol was developed. Eight rabbits were immunized with either [(5-hydroxy-2-naphthylenyl) oxy] acetic acid (2b)-BSA,[(5- hydroxy-l-naphthyleny1) oxyl acetic acid (4)-BSA, or 5-hydroxy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 different coating antigens. From this screening procedure, five assays could be developed, using two 7-KLH antisera, 3907 and 3909. ...