… for preparation of sarin-exposed human erythrocytes acetylcholinesterase using non-toxic and stable sarin analogue isopropyl p-nitrophenyl methylphosphonate and …

…, S Suzuki, H Ikegaya, K Sakurada, T Takatori

Index: Ohta, Hikoto; Ohmori, Takeshi; Suzuki, Shinichi; Ikegaya, Hiroshi; Sakurada, Koichi; Takatori, Takehiko Pharmaceutical Research, 2006 , vol. 23, # 12 p. 2827 - 2833

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Citation Number: 19

Abstract

Abstract Introduction A non-toxic and stable sarin analogue, isopropyl p-nitrophenyl methylphosphonate (INMP), was synthesized for safe preparation of sarin-exposed acetylcholinesterase (AChE). Results and Discussion This agent was stable for years, able to be handled in an ordinary laboratory without special care, and its 50% inhibitory concentration (IC 50) on 0.04 U/ml human erythrocytes AChE was 15 nM. This reagent ...