The enzymatic methylation by catechol 0-methyl transferase of 3, 4-dihydroxyacetophenone (III), 3, 4-dihydroxyphenylmethylcarbinol (V) and 3, 4-dihydroxyphenethylamine (dopamine, VI) has been studied as a function of pH. Spectrophotometric, colorimetric and chromatographic methods have been developed for the assay and resolution of 0-and p-0- methylation mixtures. Depending on the nature of the side chain, p-0-methylation has ...
