Highly efficient sequence-specific DNA interstrand cross-linking by pyrrole/imidazole CPI conjugates

…, A Narita, I Saito, H Sugiyama

Index: Bando, Toshikazu; Narita, Akihiko; Saito, Isao; Sugiyama, Hiroshi Journal of the American Chemical Society, 2003 , vol. 125, # 12 p. 3471 - 3485

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Citation Number: 27

Abstract

We have developed a novel type of DNA interstrand cross-linking agent by synthesizing dimers of a pyrrole (Py)/imidazole (Im)-diamide-CPI conjugate, ImPyLDu86 (1), connected using seven different linkers. The tetramethylene linker compound, 7b, efficiently produces DNA interstrand cross-links at the nine-base-pair sequence, 5'-PyGGC (T/A) GCC Pu-3', only in the presence of a partner triamide, ImImPy. For efficient cross-linking by 7b with ImImPy, ...