Incubations were conducted in the same manner as in the kinetic studies except each flask contained 10 mM of a low-molecularweight nucleophile (L-methionine, L-cysteine, glutathione, guanosine phosphate) or 1 mg/mL of tRNA. A saturating concentration of inactivator (N-arylhydroxamic acid) was used and preincubation times (2-20 min) were adjusted such that maximum inactivation of the enzyme occurred. Control flasks contained ...
