A “tag-and-modify” approach to site-selective protein modification

JM Chalker, GJL Bernardes…

Index: Lin, Yuya A.; Chalker, Justin M.; Floyd, Nicola; Bernardes, Goncalo J. L.; Davis, Benjamin G. Journal of the American Chemical Society, 2008 , vol. 130, # 30 p. 9642 - 9643

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Citation Number: 160

Abstract

Covalent modification can expand a protein's functional capacity. Fluorescent or radioactive labeling, for instance, allows imaging of a protein in real time. Labeling with an affinity probe enables isolation of target proteins and other interacting molecules. At the other end of this functional spectrum, protein structures can be naturally altered by enzymatic action. Protein– protein interactions, genetic regulation, and a range of cellular processes are under the ...