Bioorganic chemistry

Synthesis and Evaluation of Alternative Substrates for Arginasease

S Han, RA Moore, RE Viola

Index: Han, Shoufa; Moore, Roger A.; Viola, Ronald E. Bioorganic Chemistry, 2002 , vol. 30, # 2 p. 81 - 94

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Citation Number: 13

Abstract

Two novel carboxyl-containing arginase substrates, 4-guanidino-3-nitrobenzoic acid and 4- guanidino-2-nitrophenylacetic acid, have been synthesized and found to give enhanced catalysis and dramatically lower Km values relative to 1-nitro-3-guanidinobenzene, a substrate designed for use in a chromophoric arginase assay. To more efficiently mimic the natural substrate, a series of sulfur analogs of l-arginine were synthesized and kinetically ...