Catalytic recruitment in the inactivation of serine proteases by phosphonate esters. Recruitment of acid-base catalysis

IM Kovach, M Larson, RL Schowen

Index: Kovach, Ildiko M.; Larson, Mark; Schowen, Richard L. Journal of the American Chemical Society, 1986 , vol. 108, # 18 p. 5490 - 5495

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Citation Number: 34

Abstract

Abstract: Two new inhibitors, bis (4-nitrophenyl) propylphosphonate (NPN) and methyl 4- nitrophenyl propylphosphonate (MPN), react irreversibly with the serine proteases bovine pancreatic a-chymotrypsin (EC 3.4. 21. l), porcine pancreatic elastase (EC 3.4. 21.11), and subtilisin (EC 3.4. 21.3)(NPN also reacts with bovine pancreatic trypsin (EC 3.4. 21.4)) to liberate 1 mol of 4-nitrophenol with production of an inactive enzyme. As with many other ...