Fluorescence-labeled sphingosines as substrates of sphingosine kinases 1 and 2

…, D Mechtcheriakova, H Schmid, P Nussbaumer

Index: Ettmayer, Peter; Billich, Andreas; Baumruker, Thomas; Mechtcheriakova, Diana; Schmid, Heide; Nussbaumer, Peter Bioorganic and Medicinal Chemistry Letters, 2004 , vol. 14, # 6 p. 1555 - 1558

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Citation Number: 36

Abstract

Fluorescently labeled d-erythro-sphingosines have been successfully synthesized in 9 and 12 steps starting from commercially available Garner aldehyde. Determining the influence of the nature, position and linkage of the label on the in vitro phosphorylation rate by sphingosine kinases 1 and 2 resulted in the identification of a pyrene-and a NBD-labeled sphingosine which are both phosphorylated with efficiency comparable to the natural ...