Molecules

Design and synthesis of immunoconjugates and development of an indirect ELISA for rapid detection of 3, 5-dinitrosalicyclic acid hydrazide

YD Shen, SW Zhang, HT Lei, H Wang, ZL Xiao…

Index: Shen, Yu-Dong; Zhang, Shi-Wei; Lei, Hong-Tao; Wang, Hong; Xiao, Zhi-Li; Jiang, Yue-Ming; Sun, Yuan-Ming Molecules, 2008 , vol. 13, # 9 p. 2238 - 2248

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Citation Number: 14

Abstract

Abstract: In this study novel immunoconjugates were designed, synthesized and then used to develop a rapid, specific and sensitive indirect ELISA method to directly detect residues of 3, 5-dinitrosalicyclic acid hydrazide (DNSH), a toxic metabolite of nifursol present in chicken tissues. The hapten DNSHA was first designed and used to covalently couple to BSA to form an immunogen which was immunized to rabbits to produce a polyclonal antibody against ...