Lipase from Burkholderia cepacia (PSL-C) effectively catalyzed the kinetic resolution of both racemic trans-N, N-diallylcyclohex-4-ene-1, 2-diamine (±)-6 and its precursor trans-6- (diallylamino) cyclohex-3-enol (±)-5. The resulting optically active vicinal diamine and β- amino alcohol were converted into a precursor of oseltamivir and a cis-cyclohex-4-ene-1, 2- diamine derivative, respectively.