Various 1-(substituted)-1, 4-dihydro-6-nitro-4-oxo-7-(sub-secondary amino)-quinoline-3- carboxylic acids were synthesized from 2, 4-dichlorobenzoic acid by six step synthesis. The compounds were evaluated for antimycobacterial in vitro and in vivo against Mycobacterium tuberculosis H37Rv (MTB), multi-drug resistant Mycobacterium tuberculosis (MDR-TB) and Mycobacterium smegmatis (MC2) and also tested for the ability to inhibit the supercoiling ...