ACS Catalysis

Enantioselective hydrolysis of racemic and meso-epoxides with recombinant Escherichia coli expressing epoxide hydrolase from Sphingomonas sp. HXN-200: …

S Wu, A Li, YS Chin, Z Li

Index: Wu, Shuke; Li, Aitao; Chin, Yit Siang; Li, Zhi ACS Catalysis, 2013 , vol. 3, # 4 p. 752 - 759

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Citation Number: 10

Abstract

A unique epoxide hydrolase (SpEH) from Sphingomonas sp. HXN-200 was identified and cloned based on genome sequencing and expressed in Escherichia coli. The engineered E. coli (SpEH) showed the same selectivity and substrate specificity as the wild type strain and 172 times higher activity than Sphingomonas sp. HXN-200 for the hydrolysis of styrene oxide 1. Hydrolysis of racemic styrene oxide 1, substituted styrene oxides 3, 5–7, and N- ...