DEAE-Sephadex A 25

DEAE-Sephadex A 25 Structure
DEAE-Sephadex A 25 structure
Common Name DEAE-Sephadex A 25
CAS Number 12609-80-2 Molecular Weight N/A
Density N/A Boiling Point N/A
Molecular Formula N/A Melting Point N/A
MSDS USA Flash Point N/A

Enzymatic primer-extension with glycerol-nucleoside triphosphates on DNA templates.

PLoS ONE 4(3) , e4949, (2009)

Glycerol nucleic acid (GNA) has an acyclic phosphoglycerol backbone repeat-unit, but forms stable duplexes based on Watson-Crick base-pairing. Because of its structural simplicity, GNA is of particular interest with respect to the possibility of evolving func...

Low-cost production of 6G-fructofuranosidase with high value-added astaxanthin by Xanthophyllomyces dendrorhous.

Bioresour. Technol. 104 , 660-667, (2012)

The effects of medium composition and culture conditions on the production of (6)G-fructofuranosidase with value-added astaxanthin were investigated to reduce the capital cost of neo-fructooligosaccharides (neo-FOS) production by Xanthophyllomyces dendrorhous...

Factors affecting the resolution of dl-menthol by immobilized lipase-catalyzed esterification in organic solvent.

J. Agric. Food Chem. 50(2) , 262-5, (2002)

Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yi...

A new Cu(II)- 5-(4-sulphophenylazo)-8-aminoquinoline complex used for copper determination in presence of gold and silver in water and mineral samples

Talanta 74(1) , 110-8, (2007)

In this work, a characterization of reagent chromophere 5-(4-sulphophenylazo)-8-aminoquinoline [SPA] by IR and 1H RMN was carried out and a p K a value of 3.55 ± 0.03 was found as well. An 1:2 stoichiometry for the Cu(II)-SPA complex was determined at pH 9 by...

Physicochemical and chromatographic evaluation of benzhydrylamine-resin as an anion exchanger solid support.

J. Biochem. Biophys. Methods 57(1) , 75-83, (2003)

Benzhydrylamine-resin (BHAR), a copolymer of styrene-divinylbenzene containing phenylmethylamine groups, used as solid support for peptide synthesis, was examined regarding physicochemical and anion exchanger chromatographic properties. The greater the ionic ...

Hypolipidemic effects of orally administered dextran and cellulose anion exchangers in cockerels and dogs.

J. Lipid Res. 8(1) , 24-9, (1967)

Various cellulose and dextran anion exchangers bind bile salts in vitro under conditions of pH and ionic strength resembling those in the lumen of the small intestine. Of these substances, diethylaminoethyl (DEAE) cellulose, guanidoethyl cellulose, and DEAE S...

RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components.

Mol. Cell. Biol. 6(4) , 1058-1064, (1986)

A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA. This mitochondrial R...

Pregnenolone sulfate and cortisol induce secretion of acyl-CoA-binding protein and its conversion into endozepines from astrocytes.

J. Biol. Chem. 285(28) , 21359-21365, (2010)

Acyl-CoA-binding protein (ACBP) functions both intracellularly as part of fatty acid metabolism and extracellularly as diazepam binding inhibitor, the precursor of endozepine peptides. Two of these peptides, ODN and TTN, bind to the GABA(A) receptor and modul...

In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.

Exp. Cell Res. 139(2) , 401-404, (1982)

Isolation of glutamate-binding proteins from rat and bovine brain synaptic membranes and immunochemical and immunocytochemical characterization.

Neuroscience 46(4) , 793-806, (1992)

We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified...