Go 6983 structure
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Common Name | Go 6983 | ||
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CAS Number | 133053-19-7 | Molecular Weight | 442.510 | |
Density | 1.3±0.1 g/cm3 | Boiling Point | 709.0±60.0 °C at 760 mmHg | |
Molecular Formula | C26H26N4O3 | Melting Point | N/A | |
MSDS | Chinese USA | Flash Point | 382.6±32.9 °C | |
Symbol |
GHS07 |
Signal Word | Warning |
Use of Go 6983Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively. |
Name | 3-[1-[3-(dimethylamino)propyl]-5-methoxyindol-3-yl]-4-(1H-indol-3-yl)pyrrole-2,5-dione |
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Synonym | More Synonyms |
Description | Go 6983 is a pan-PKC inhibitor against for PKCα, PKCβ, PKCγ, PKCδ and PKCζ with IC50 of 7 nM, 7 nM, 6 nM, 10 nM and 60 nM, respectively. |
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Related Catalog | |
Target |
PKCγ:6 nM (IC50) PKCα:7 nM (IC50) PKCβ:7 nM (IC50) PKCδ:10 nM (IC50) PKCζ:60 nM (IC50) PKCμ:20000 nM (IC50) |
In Vitro | Go 6983 inhibits PKCμ with IC50 of 20 μM, and the pther PKC isoenzymes can be suppressed by Go 6983 with IC50 values from 7 to 60 nM[1]. Go 6983 (100 nM) significantly reduces PMN adherence to the endothelium and infiltration into the myocardium compared with I/R + PMN hearts, and significantly inhibits superoxide release from PMNs by 90 +/- 2% in rat hearts[2]. Go 6983 (200 nM) has a reduced cardioprotective effect compared with the cardioprotective Go 6983 concentrations (50 and 100 nM) despite inhibiting PMN superoxide release by 99%[3]. |
Kinase Assay | Phosphorylation reactions are carried out in a total volume of 100 μL, containing buffer C (50 mM Tris-HCl, pH 7.5, 10 mM β-mercaptoethanol), 4 mM MgCl2, 10 μg PS, 100 nM TPA, 5 μL of a Sf158 cell extract as a source of recombinant PKCμ or of Sf9 cell extracts as a source of other recombinant PKC isoenzymes, 10 μg of syntide 2 as substrate, and 35 μM ATP containing 1 μCi [γ-32P]ATP. In some experiments, PS and TPA are omitted or various inhibitors at concentrations indicated in the text are added. After incubation for 10 min at 30°C, the reaction is terminated by transferring 50 μL of the assay mixture onto a 20 mm square piece of phosphocellulose paper, which is washed 3 times in deionized water and twice in acetone. The radioactivity on each paper is determined by liquid scintillation counting. |
References |
Density | 1.3±0.1 g/cm3 |
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Boiling Point | 709.0±60.0 °C at 760 mmHg |
Molecular Formula | C26H26N4O3 |
Molecular Weight | 442.510 |
Flash Point | 382.6±32.9 °C |
Exact Mass | 442.200500 |
PSA | 79.36000 |
LogP | 3.80 |
Appearance of Characters | red |
Vapour Pressure | 0.0±2.3 mmHg at 25°C |
Index of Refraction | 1.670 |
Storage condition | −20°C |
Water Solubility | DMSO: 20 mg/mL, clear, red |
Symbol |
GHS07 |
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Signal Word | Warning |
Hazard Statements | H315-H319-H335 |
Precautionary Statements | P261-P305 + P351 + P338 |
Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
Hazard Codes | Xi |
Risk Phrases | 36/37/38 |
Safety Phrases | 26-36 |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
HSPB1 as a novel regulator of ferroptotic cancer cell death.
Oncogene 34(45) , 5617-25, (2015) Ferroptosis is an iron-dependent form of non-apoptotic cell death, but its molecular mechanism remains largely unknown. Here, we demonstrate that heat shock protein beta-1 (HSPB1) is a negative regula... |
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High-throughput protease activity cytometry reveals dose-dependent heterogeneity in PMA-mediated ADAM17 activation.
Integr. Biol. (Camb.) 7 , 513-24, (2015) As key components of autocrine signaling, pericellular proteases, a disintegrin and metalloproteinases (ADAMs) in particular, are known to impact the microenvironment of individual cells and have sign... |
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Downregulation of vimentin in macrophages infected with live Mycobacterium tuberculosis is mediated by Reactive Oxygen Species.
Sci. Rep. 6 , 21526, (2016) Mycobacterium tuberculosis persists primarily in macrophages after infection and manipulates the host defence pathways in its favour. 2D gel electrophoresis results showed that vimentin, an intermedia... |
3-{1-[3-(Dimethylamino)propyl]-5-methoxy-1H-indol-3-yl}-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione |
3-[1-[3-(Dimethylamino)propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione |
2-(1-(3-dimethylaminopropyl)-5-methoxyindol-3-yl)-3-(1H-indol-3-yl)maleimide |
go 6983 |
1H-Pyrrole-2,5-dione, 3-[1-[3-(dimethylamino)propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)- |