H-Ala-Gly-Glu-Gly-Leu-Ser-Ser-Pro-Phe-Trp-Ser-Leu-Ala-Ala-Pro-Gln-Arg-Phe-NH2

Names

[ Name ]:
H-Ala-Gly-Glu-Gly-Leu-Ser-Ser-Pro-Phe-Trp-Ser-Leu-Ala-Ala-Pro-Gln-Arg-Phe-NH2

[Synonym ]:
ALA-GLY-GLU-GLY-LEU-SER-SER-PRO-PHE-TRP-SER-LEU-ALA-ALA-PRO-GLN-ARG-PHE-NH2
ALA-GLY-GLU-GLY-LEU-SER-SER-PRO-PHE-TRP-SER-LEU-ALA-ALA-PRO-GLN-ARG-PHE AMIDE
A18Famide
A-18-F-NH2
MFCD00076703

Biological Activity

[Description]:

Neuropeptide AF (cattle), an amidated octadecapeptide, is RFamide neuropeptide. Neuropeptide AF (cattle) acts as a ligand of Mas-related gene receptor A4 (MrgprA4) (Mas-related G-protein-coupled Receptor (MRGPR)) (EC50 of ~60 nM) and MrgprC11 (EC50 of ~300 nM). Neuropeptide AF (cattle) also activate to the G protein-coupled receptors NPFF1 (Neuropeptide Y Receptor) (EC50 of ~25-325 nM) and NPFF2 (EC50 of ~1-5 nM). Neuropeptide AF (cattle) shows anti-opiate and related pain modulation effects[1][2].

[Related Catalog]:

Research Areas >> Neurological Disease

Signaling Pathways >> GPCR/G Protein >> Neuropeptide Y Receptor

[In Vitro]

Neuropeptide AF (NPAF) (50, 100, 150, 300, and 600nM) induces a piecemeal degranulation in bone marrow-derived mucosal mast cells (BMMCs). Neuropeptide AF can be considered as a novel modulator of BMMC activity in the neuro-immune communication in the gastrointestinal tract[1].

[In Vivo]

Neuropeptide AF (cattle) (0.25 μg/2 μL; i.c.v.; 30 min prior to the tests) decreases the immobility time and increases the climbing and swimming times in modified mouse forced swimming test (mFST)[2]. Animal Model: Male CFLP mice (25-28 g)[2] Dosage: 0.25 μg/2 μL Administration: i.c.v.; 30 min prior to the tests Result: Decreased the immobility time and increased the climbing and swimming times.

[References]

[1]. Nada Abdellah, et al. Neuropeptide AF Induces Piecemeal Degranulation in Murine Mucosal Mast Cells: A New Mediator in Neuro-Immune Communication in the Intestinal Lamina Propria? Anat Rec (Hoboken). 2018 Jun;301(6):1103-1114.

[2]. Miklós Palotai, et al. Neuropeptide AF induces anxiety-like and antidepressant-like behavior in mice. Behav Brain Res. 2014 Nov 1;274:264-9.

Chemical & Physical Properties

[ Molecular Formula ]:
C₈₉H₁₃₀N₂₄O₂₄

[ Molecular Weight ]:
1920.13000

[ Exact Mass ]:
1918.97000

[ PSA ]:
765.00000

[ LogP ]:
1.07870