ZK 756326
Names
[ CAS No. ]:
874911-96-3
[ Name ]:
ZK 756326
[Synonym ]:
2-[2-[4-[(3-Phenoxyphenyl)methyl]-1-piperazinyl]ethoxy]ethanol
ZK 756326
MFCD09038571
Biological Activity
[Description]:
ZK756326 is a nonpeptide chemokine receptor agonist for the CC chemokine receptor CCR8.
[Related Catalog]:
[Target]
IC50: 1.8 μM (CCR8)[1]
[Cell Assay]
U87 MG cells expressing CCR8 are plated on poly-D-lysine-coated black 96-well plates at 10,000 cells/well and are cultured overnight. Cells are then loaded with Calcium 3, a Ca2+-sensitive non-wash fluorescence dye, for 60 min at 37°C in Hanks' balanced salts solution containing 20 mM HEPES, 3.2 mM CaCl2, 1% (v/v) fetal bovine serum, and 2.5 mM probenecid. Changes in intracellular free-Ca2+ concentration are measured with Fluorometric Imaging Plate Reader (FLIPR 3) immediately after the addition of agonist at room temperature. Cross-desensitization experiments are performed by a first addition of the agonist (CCL1 at 30 nM or ZK 756326 at 3 μM), immediately followed by a second addition of 100 nM CCL1[1].
[References]
Chemical & Physical Properties
[ Density]:
1.142±0.06 g/cm3
[ Molecular Formula ]:
C21H28N2O3
[ Molecular Weight ]:
356.46
[ Exact Mass ]:
428.16300
[ PSA ]:
45.17000
[ LogP ]:
4.08520
[ Storage condition ]:
-20℃
[ Water Solubility ]:
Slightly soluble (3 g/L) (25 ºC)