8-Azido-ATP

8-Azido-ATP, a photoreactable nucleotide analog, is useful for the identification of proteins, such as DNA-dependent RNA polymerase[1].

Research Areas >> Metabolic Disease

Signaling Pathways >> Membrane Transporter/Ion Channel >> Potassium Channel

8-Azido-ATP can be used to identify the viral RNA polymerase on the basis of the ability of the analog to inhibit transcription activity associated with rotavirus particles on exposure to UV light[1]. 8-Azido-ATP (azido-ATP) decreases viral transcription in a dose-dependent manner[1]. 8-Azido-ATP blocks Kir6.2ΔC26 currents rather less potently than ATP, half-maximal inhibition (Ki) occurring at 2.8 ± 0.4 mM (n = 6) compared with 172 ± 7 mM for ATP (n = 6). The Hill coefficients were 0.9 ± 0.2 for 8-azido-ATP and 1.3 ± 0.1 for ATP[2].

[1]. S Valenzuela, et al. Photoaffinity labeling of rotavirus VP1 with 8-azido-ATP: identification of the viral RNA polymerase. J Virol. 1991 Jul;65(7):3964-7.

[2]. K Tanabe, et al. Direct photoaffinity labeling of the Kir6.2 subunit of the ATP-sensitive K+ channel by 8-azido-ATP. J Biol Chem. 1999 Feb 12;274(7):3931-3.