Ginsenoside Rk1

Ginsenoside Rk1 is a unique component created by processing the ginseng plant (mainly Sung Ginseng, SG) at high temperatures[1].Ginsenoside Rk1 has anti-inflammatory effect, suppresses the activation of Jak2/Stat3 signaling pathway and NF-κB[2].Ginsenoside Rk1 has anti-tumor effect, antiplatelet aggregation activities, anti-insulin resistance, nephroprotective effect, antimicrobial effect, cognitive function enhancement, lipid accumulation reduction and prevents osteoporosis[1].Ginsenoside Rk1 induces cell apoptosis by triggering intracellular reactive oxygen species (ROS) generation and blocking PI3K/Akt pathway[3].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> JAK/STAT Signaling >> JAK

Signaling Pathways >> Epigenetics >> JAK

Signaling Pathways >> Stem Cell/Wnt >> JAK

Signaling Pathways >> PI3K/Akt/mTOR >> PI3K

Research Areas >> Inflammation/Immunology

Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM[2]. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner[2]. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control[3]. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells[3]. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation[3]. RT-PCR[2] Cell Line: RAW264.7 cells Concentration: 10 μM, 20 μM, 40 μM Incubation Time: 6 hours Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. Western Blot Analysis[2] Cell Line: RAW264.7 cells Concentration: 10 μM, 20 μM, 40 μM Incubation Time: 6 hours Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells Cell Viability Assay[3] Cell Line: MDA-MB-231 cells Concentration: 0 μM, 40 μM, 80 μM, 120 μM Incubation Time: 48 hours Result: Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. Cell Cycle Analysis[3] Cell Line: MDA-MB-231 cells Concentration: 0 μM, 40 μM, 80 μM, 120 μM Incubation Time: 24 hours Result: Induced G0/G1 phase arrest. Apoptosis Analysis[3] Cell Line: MDA-MB-231 cells Concentration: 0 μM, 40 μM, 80 μM, 120 μM Incubation Time: 24 hours Result: Induced apoptosis in MDA-MB-231 cells.

[1]. Elshafay A, et al. Ginsenoside Rk1 bioactivity: a systematic review. PeerJ. 2017 Nov 17;5:e3993.

[2]. Yu Q,et al. Ginsenoside Rk1 suppresses pro-inflammatory responses in lipopolysaccharide-stimulated RAW264.7 cells by inhibiting the Jak2/Stat3 pathway. Chin J Nat Med. 2017 Oct;15(10):751-757.

[3]. Hong Y, et al. Ginsenoside Rk1 induces cell cycle arrest and apoptosis in MDA-MB-231 triple negative breast cancer cells. Toxicology. 2019 Apr 15;418:22-31.