Solvent Black 3

Names

[ Name ]:
Solvent Black 3

[Synonym ]:
(2,2-dimethyl-1,3-dihydroperimidin-6-yl)-(4-phenyldiazenylnaphthalen-1-yl)diazene
MFCD00006919
2,2-dimethyl-6-[(E)-{4-[(E)-phenyldiazenyl]naphthalen-1-yl}diazenyl]-2,3-dihydro-1H-perimidine
Sudan Black B,high purity biological stain
Fat Black HB
Sudan Black B,certified
2,2-Dimethyl-6-[(E)-{4-[(E)-phenyldiazenyl]-1-naphthyl}diazenyl]-2,3-dihydro-1H-perimidine
SOLVENT BLACK 3
1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-[(E)-2-[4-[(E)-2-phenyldiazenyl]-1-naphthalenyl]diazenyl]-
2,3-dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthyl]azo]perimidine
Ceres Black BN
EINECS 224-087-1
Sudan Black B

Biological Activity

[Description]:

Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Sudan Black B works also in frozen material, and therefore can be complementarily used with SA-β-gal when investigating senescence. Sudan Black B is a highly lipophilic agent that exhibits high affi nity to the lipid compartment of lipofuscin. The positive Sudan Black B-lipofuscin reaction reveals blue-black intracellular granules in cells and frozen tissues and brown to black granules in FFPE tissues under the light microscope.

[References]

[1]. Konstantinos Evangelou, et al. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. Methods Mol Biol. 2017;1534:111-119.

Chemical & Physical Properties

[ Density]:
1.3±0.1 g/cm3

[ Boiling Point ]:
726.0±60.0 °C at 760 mmHg

[ Melting Point ]:
120-124 °C(lit.)

[ Molecular Formula ]:
C₂₉H₂₄N₆

[ Molecular Weight ]:
456.541

[ Flash Point ]:
392.9±32.9 °C

[ Exact Mass ]:
456.206238

[ PSA ]:
73.50000

[ LogP ]:
7.51

[ Vapour Pressure ]:
0.0±2.4 mmHg at 25°C

[ Index of Refraction ]:
1.702

[ Storage condition ]:
Store at RT.

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SD4431500

CHEMICAL NAME :: 1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl )azo)-

CAS REGISTRY NUMBER :: 4197-25-5

LAST UPDATED :: 199712

DATA ITEMS CITED :: 4

MOLECULAR FORMULA :: C29-H24-N6

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 63 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010) Volume(issue)/page/year: NX#04918 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M2167 No. of Facilities: 601 (estimated) No. of Industries: 10 No. of Occupations: 10 No. of Employees: 4471 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M2167 No. of Facilities: 1141 (estimated) No. of Industries: 15 No. of Occupations: 24 No. of Employees: 19262 (estimated) No. of Female Employees: 13466 (estimated)

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
F: Flammable;Xi: Irritant;

[ Risk Phrases ]:
11-36/37/38

[ Safety Phrases ]:
S24/25-S16

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
SD4431500

[ HS Code ]:
32041900

Customs

[ HS Code ]: 32041900

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