bleomycin A6

Boanmycin is an antibiotic with antitumor activity that induces cellular senescence and apoptosis[1][2][3].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Boanmycin (50-125 μg/mL, 0.5-36 h) can induce apoptosis and block the cell cycle in the G2/M phase in Eca-109 cells[2]. Boanmycin (6.7 µg/mL, 24 h) induces cell senescence[3]. Cell Viability Assay[3] Cell Line: Human lung fibroblast cell line IMR90, mouse primary osteoblasts (OBs) Concentration: 6.7 µg/mL Incubation Time: 24 hours Result: Showed a 90% positive number of SA-βgal stained cells in IMR90 cells and 95% in OBs compared to 30% in the control group. RT-PCR[3] Cell Line: Mouse primary osteoblasts (OBs) Concentration: 6.7 µg/mL Incubation Time: 24 hours Result: Increased senescence-associated secretory phenotype (SASP) factor IL-6 expression up to six-fold.

Boanmycin (BAM) (i.p., 10 or 15 mg/kg, twice a week, 5 weeks)can effectively inhibit the growth of tumors in BALB/c nude mice with HT-29, Hce-8693 or CT-26 cells[1]. Animal Model: BALB/c nude mice with HT-29 or Hce-8693 cells[1] Dosage: 10 or 15 mg/kg Administration: Intraperitoneal injection; twice a week; 5 weeks Result: Significantly inhibited tumor growth by 89% at 10 mg/kg and by 92% at 15 mg/kg in mice with HT-29 model, also inhibited tumor growth by 90% at 10 mg/kg and by 92% at 15 mg/kg in mice with Hce-8693 model. Animal Model: BALB/c nude mice with CT-26 cells[1] Dosage: 10 mg/kg Administration: Intramuscular injection; every second day; 10 times Result: Inhibited subcutaneous tumor growth by 88% and cecal tumor by 99%.

[1]. Y C Deng, et al. Activity of boanmycin against colorectal cancer. World J Gastroenterol. 2001 Feb;7(1):93-7.

[2]. Hao Tang, et al. Effect of boanmycin on apoptosis and cell cycle of human esophageal cancer(Eca-109) cells]. Ai Zheng. 2002 Aug;21(8):855-9.

[3]. Peng Chen, et al. The chemotherapeutic drug boanmycin induces cell senescence and senescence-associated secretory phenotype factors, thus acquiring the potential to remodel the tumor microenvironment. Anticancer Drugs. 2016 Feb;27(2):84-8.

MOLECULAR FORMULA :

C60-H96-N20-O21-S2

MOLECULAR WEIGHT :

1497.88

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intraperitoneal

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

102 mg/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

REFERENCE :

ZKZAEY Zhongguo Kangshengsu Zazhi. Chinese Journal of Antibiotics. (Zhongguo Kangshengsu Zazhi Bianjibu, 9 Shanbanqiaolu, Chenqdu, 610051 Peop. Rep. China) V.13- 1988- Volume(issue)/page/year: 15,453,1990

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Subcutaneous

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

80330 ug/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

REFERENCE :

ZKZAEY Zhongguo Kangshengsu Zazhi. Chinese Journal of Antibiotics. (Zhongguo Kangshengsu Zazhi Bianjibu, 9 Shanbanqiaolu, Chenqdu, 610051 Peop. Rep. China) V.13- 1988- Volume(issue)/page/year: 15,453,1990

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

97780 ug/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

REFERENCE :

ZKZAEY Zhongguo Kangshengsu Zazhi. Chinese Journal of Antibiotics. (Zhongguo Kangshengsu Zazhi Bianjibu, 9 Shanbanqiaolu, Chenqdu, 610051 Peop. Rep. China) V.13- 1988- Volume(issue)/page/year: 15,453,1990

TYPE OF TEST :

LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :

Intramuscular

SPECIES OBSERVED :

Rodent - mouse

DOSE/DURATION :

70530 ug/kg

TOXIC EFFECTS :

Details of toxic effects not reported other than lethal dose value

REFERENCE :

ZKZAEY Zhongguo Kangshengsu Zazhi. Chinese Journal of Antibiotics. (Zhongguo Kangshengsu Zazhi Bianjibu, 9 Shanbanqiaolu, Chenqdu, 610051 Peop. Rep. China) V.13- 1988- Volume(issue)/page/year: 15,453,1990 ** OTHER MULTIPLE DOSE TOXICITY DATA **

TYPE OF TEST :

TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :

Intravenous

SPECIES OBSERVED :

Mammal - dog

DOSE/DURATION :

24500 ug/kg/7W-I

TOXIC EFFECTS :

Kidney, Ureter, Bladder - changes in tubules (including acute renal failure, acute tubular necrosis) Nutritional and Gross Metabolic - weight loss or decreased weight gain Related to Chronic Data - death

REFERENCE :

ZKZAEY Zhongguo Kangshengsu Zazhi. Chinese Journal of Antibiotics. (Zhongguo Kangshengsu Zazhi Bianjibu, 9 Shanbanqiaolu, Chenqdu, 610051 Peop. Rep. China) V.13- 1988- Volume(issue)/page/year: 15,453,1990