Emodin 6-O-beta-D-glucoside

Emodin-6-O-β-D-glucoside (Glucoemodin) is an active compound from Reynoutria japonica. Emodin-6-O-β-D-glucoside shows potent anti-inflammatory and barrier protective effects. Emodin-6-O-β-D-glucoside can be used for the research of diabetic complications and atherosclerosis[1].

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

Research Areas >> Metabolic Disease

Emodin-6-O-β-D-glucoside (0~20 μM; 24 hours; HUVECs) does not affect cell viability[1]. Emodin-6-O-β-D-glucoside (0~10 μM; 6 hours; HUVECs) results in a dose dependent decrease in high glucose-mediated membrane disruption, inhibits high glucose-induced increase expression of VCAM-1, ICAM-1 and E-selectin and results in decreased expression levels of high glucose-induced MCP-1 and IL-8 mRNA[1]. Emodin-6-O-β-D-glucoside (10 μM; 6 hours; HUVECs) results in a decrease in the number of THP-1 cells adhering to high glucose-induced HUVECs[1]. Emodin-6-O-β-D-glucoside pretreatment significantly inhibits high-glucose-induced ROS formation and p65 NF-κB expression levels increase[1]. Cell Viability Assay[1] Cell Line: HUVECs Concentration: 0~20 μM Incubation Time: 24 hours Result: Did not affect cell viability. Western Blot Analysis[1] Cell Line: HUVECs Concentration: 0~10 μM Incubation Time: 6 hours Result: Inhibited high glucose-induced increase expression of VCAM-1, ICAM-1 and E-selectin. RT-PCR[1] Cell Line: HUVECs Concentration: 0~10 μM Incubation Time: 6 hours Result: Resulted in decreased expression levels of high glucose-induced MCP-1 and IL-8 mRNA. Immunofluorescence[1] Cell Line: HUVECs Concentration: 10 μM Incubation Time: 6 hours Result: Resulted in a decrease in the number of THP-1 cells adhering to high glucose-induced HUVECs.

Emodin-6-O-β-D-glucoside (0~9 mg/mouse; i.v.) markly inhibits peritoneal leakage of dye induced by high glucose[1]. Animal Model: Mouse[1] Dosage: 0~9 mg/mouse Administration: I.v. Result: Markly inhibited peritoneal leakage of dye induced by high glucose.

[1]. Lee W, et al. Emodin-6-O-β-D--glucoside inhibits high-glucose-induced vascular inflammation. Inflammation. 2014;37(2):306-313.