Xylene Cyanole F

Names

[ Name ]:
Xylene Cyanole F

[Synonym ]:
Sodium 4-{(Z)-[4-(ethylamino)-3-methylphenyl][(4Z)-4-(ethyliminio)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl}benzene-1,3-disulfonate
Xylene Cyanol FF
Acid Blue 147
XYLENE CYANOL
CYANOL BLUE
EINECS 224-728-5
Sodium 4-{(Z)-[4-(ethylamino)-3-methylphenyl][(4Z)-4-(ethyliminio)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl}-1,3-benzenedisulfonate
1,3-Benzenedisulfonic acid, 4-((4-(ethylamino)-3-methylphenyl)(4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene)methyl)-, monosodium salt
Xylene Cyanole F
Sodium hydrogen 4-((4-(ethylamino)-m-tolyl)(4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene)methyl)benzene-1,3-disulphonate
CYANOL
Blue 147
Acid blue 147,Cyanol FF,XC
1,3-Benzenedisulfonic acid, 4-[(Z)-[4-(ethylamino)-3-methylphenyl][(4Z)-4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-, sodium salt (1:1)
MFCD00040657
CYANOL FF

Biological Activity

[Description]:

Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested[1][2].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

Xylene Cyanol FF 能够在不同聚丙烯酰胺凝胶电泳示踪 DNA。操作如下[1]： 1.用于变性凝胶。（1）配制凝胶：10% 的丙烯酰胺 (19:1，丙烯酰胺：双丙烯酰胺)，以及 8.3 M urea。使用条件为 55 ℃。（2）配制电泳缓冲液：89 mM Tris，HCl，pH 8.0，89 mM 硼酸，2 mM EDTA (TBE)。（3）配制上样缓冲液：10 mM NaOH，1 mM EDTA，以及 0.1% Xylene Cyanol FF。（4）凝胶在 IBI 型号 STS 45 电泳装置上以 70 W (50 V/cm，恒定功率) 运行，或在 Hoefer SE 600 电泳装置上以 60 °C (31 V/cm，恒定电压) 运行。（5）将凝胶置于 Whatman 3MM 纸上干燥并在 X 射线胶片上曝光。曝光时间长达 15 小时。2.用于变性凝胶。（1）配制凝胶：含有 8% 的丙烯酰胺 (19:1，丙烯酰胺：双丙烯酰胺)。（2）配制 DNA 悬液：含有 40 mM TrisβHCl，pH 8.0、20 mM 乙酸、2 mM EDTA 和 12.5 mM 乙酸镁 (TAEMg)。（3）煮沸 DNA 悬液并缓慢冷却至 16 °C。（4）配制染色液：含有 TAEMg、50% 甘油、0.02% 溴酚蓝和 0.02% Xylene Cyanol FF。用该染色液将样品调至 10 µL 的最终体积。（5）凝胶在 Hoefer SE-600 凝胶电泳装置上以 11 V/cm 的电压在 16 °C 下运行，并暴露于 X 射线胶片长达 15 小时或用 Stainsall (HY-D0987) 染料染色。

[References]

[1]. Li X, et al. Antiparallel DNA double crossover molecules as components for nanoconstruction. Journal of the American Chemical Society, 1996, 118(26): 6131-6140.

[2]. Cai L, et al. Determination of iron and aluminum based on the catalytic effect on the reaction of xylene cyanol FF with hydrogen peroxide and potassium periodate[J]. Journal of the Brazilian Chemical Society, 2011, 22: 1987-1992.

Chemical & Physical Properties

[ Melting Point ]:
295°C

[ Molecular Formula ]:
C₂₅H₂₇N₂NaO₆S₂

[ Molecular Weight ]:
538.611

[ Exact Mass ]:
538.120850

[ PSA ]:
152.72000

[ LogP ]:
6.59110

[ Storage condition ]:
Store at RT.

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S36-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
32041900

Customs

[ HS Code ]: 32041900

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