c16 ceramide

C16-Ceramide is a natural small molecule activating p53 through the direct and selective binding[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Apoptosis >> MDM-2/p53

Human Endogenous Metabolite

C16-Ceramide interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide in the cell[1]. C16-Ceramide (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2]. C16-Ceramide (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2]. C16-ceramide (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-ceramide down-regulates Mdm2 expression via Btf[2]. Cell Viability Assay[2] Cell Line: HCT116 cells Concentration: 2.5, 5, 10, 12, 20, 50 µM Incubation Time: 0-48 h Result: Strongly decreased cell viability in a time- and concentration-dependent manner. Western Blot Analysis[2] Cell Line: HCT116 cells Concentration: 12 μM Incubation Time: 1, 3 and 6 h Result: Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion alsopartially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.