FDP

Fluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product[1][2][3].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product[1]. Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays[2]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). ELISA with capillary immunosensor (single-step ELISA)[3]: 1. Put various concentrations of antigen in Tris buffer 1 containing 1% BSA into capillary immunosensor. 2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe. 3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.

[1]. Murakami Y, et al. On-chip capillary electrophoresis for alkaline phosphatase testing. Biosens Bioelectron. 2001 Dec;16(9-12):1009-14.

[2]. Gilmartin AG, et al. Allosteric Wip1 phosphatase inhibition through flap-subdomain interaction. Nat Chem Biol. 2014 Mar;10(3):181-7.

[3]. Tsutsumi E, et al. Single-step sandwich immunoreaction in a square glass capillary immobilizing capture and enzyme-linked antibodies for simplified enzyme-linked immunosorbent assay. Anal Sci. 2012;28(1):51-6.