Amyloid β-Protein (1-42) (mouse, rat)

Names

[ CAS No. ]:
166090-74-0

[ Name ]:
Amyloid β-Protein (1-42) (mouse, rat)

[Synonym ]:
bap 1-42
asp-ala-glu-phe-arg-his-asp-ser-gly-tyr-glu-val-his-his-gln-lys-leu-val-phe-phe-ala-glu-asp-val-gly-ser-asn-lys-gly-ala-ile-ile-gly-leu-met-val-gly-gly-val-val-ile-ala
predominant amyloid b-protein fragment
beta-amyloid peptide (1-40), mouse, rat
asp-ala-glu-phe-gly-his-asp-ser-gly-phe-glu-val-arg-his-gln-lys-leu-val-phe-phe-ala-glu-asp-val-gly-ser-asn-lys-gly-ala-ile-ile-gly-leu-met-val-gly-gly-val-val-ile-ala
beta-amyloid peptide (1-40), rat
beta-amyloid, sodium salt
beta-amyloid peptide (1-42), rat
β-Amyloid (1-42), rat

Biological Activity

[Description]:

β-Amyloid (1-42), rat is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.

[Related Catalog]:

Signaling Pathways >> Neuronal Signaling >> Amyloid-β
Peptides
Research Areas >> Neurological Disease

[Target]

Amyloid-β[1]


[In Vitro]

β-Amyloid (1-42), rat shows cytotoxic effect on the hippocampal slices at 20 μM[1]. β-Amyloid (1-42), rat causes morphological changes in NGF-induced PC12 cells, induces formed cell processes to retract in differentiated cells and affect the expression of exons 2/3 in both undifferentiated and differentiated cells[2].

[Cell Assay]

After treating the hippocampal slices with 20 μM β-Amyloid (1-42) for 4 h, supernatant is replaced by fresh H-ASCF/3 (0.9 mL/chamber) to which 0.1 mL MTT stock solution (5 mg/mL H-ACSF/3) is added (MTT final concentration: 0.5 mg/mL). The chamber is left to rest for 15 min without carboxygenation. To stop further reduction of MTT, the medium (H-ACSF/3) is removed. The slices are transferred into 96-well plate, then pure DMSO (100 μL/slice/well) is added for dissolving formazane from the slices. (30 min in a 96-well plate). Then 70 μL DMSO solution from each slice (well) is transferred into another 96-well plate. The optical density (OD) of the dissolved formazane is measured at 550 and 620 nm[1].

[References]

[1]. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5.

[2]. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34.


[Related Small Molecules]

FPS-ZM1 | Amyloid β-Protein (25-35) trifluoroacetate salt | Ginsenoside Rg3 | Geniposide | Ginsenoside Rg1 | β-Amyloid-42 | Semagacestat(LY450139) | Edonerpic maleate | Notoginsenoside R1 | Latrepirdine | Frentizole | Ginsenoside Re | Ginsenoside Rg2

Chemical & Physical Properties

[ Molecular Formula ]:
C199H307N53O59S

[ Molecular Weight ]:
4418.017

[ Exact Mass ]:
4417.244

Related Compounds