NHS-5(6)Carboxyrhodamine

Names

[ Name ]:
NHS-5(6)Carboxyrhodamine

[Synonym ]:
1-({[3',6'-Bis(dimethylamino)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl]carbonyl}oxy)-2,5-pyrrolidinedione
(2,5-dioxopyrrolidin-1-yl) 3',6'-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
2,5-Pyrrolidinedione, 1-[[[3',6'-bis(dimethylamino)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]-

Biological Activity

[Description]:

NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

A general characteristic for all of the NHS-5(6)Carboxyrhodamine/ConA samples is that the 556 nm peak is significantly larger relative to the 520 nm peak[1]. Dye/protein ratios for NHS-5(6)Carboxyrhodamine under native conditions are close to the ratios obtained under denaturing conditions[1].

[References]

[1]. D L Meadows, et al. Determining the extent of labeling for tetramethylrhodamine protein conjugates. J Immunol Methods. 1991 Oct 25;143(2):263-72.

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
759.4±70.0 °C at 760 mmHg

[ Molecular Formula ]:
C₂₉H₂₅N₃O₇

[ Molecular Weight ]:
527.525

[ Flash Point ]:
413.1±35.7 °C

[ Exact Mass ]:
527.169250

[ PSA ]:
105.69000

[ LogP ]:
2.36

[ Vapour Pressure ]:
0.0±2.6 mmHg at 25°C

[ Index of Refraction ]:
1.710

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
F: Flammable;

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Topological stability and self-association of a completely hydrophobic model transmembrane helix in lipid bilayers.

Biochemistry 41 , 3073-3080, (2002)

Investigation of interactions between hydrophobic model peptides and lipid bilayers is perhaps the only way to elucidate the principles of the folding and stability of membrane proteins (White, S. H.,...

Two-dimensional fluorescence intensity distribution analysis: theory and applications.

Biophys. J. 78 , 1703-1713, (2000)

A method of sample analysis is presented which is based on fitting a joint distribution of photon count numbers. In experiments, fluorescence from a microscopic volume containing a fluctuating number ...

E. Koller

Appl. Fluoresc. Technol. 3 , 20, (1991)

Related Compounds

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