TBK1/IKKε-IN-4
Names
[ CAS No. ]:
1381930-17-1
[ Name ]:
TBK1/IKKε-IN-4
[Synonym ]:
1H-Pyrazolo[3,4-d]pyrimidin-6-amine, N-[3,4-dimethoxy-5-[3-(4-methyl-1-piperazinyl)propoxy]phenyl]-1-(4-methoxyphenyl)-
N-{3,4-Dimethoxy-5-[3-(4-methyl-1-piperazinyl)propoxy]phenyl}-1-(4-methoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-6-amine
Biological Activity
[Description]:
TBK1-IKKε inhibitor II is a potent, selective dual inhibitor of TBK1/IKKε with IC50 of 13 nM/59 nM, respectively; displays 100- to 1000-fold less activity against other tested protein kinases including PDK1, PI3K family members and mTOR; inhibits LPS-induced expression of IFNβ (IC50=62 nM), and the IFNβ target genes IP10 (IC50=78 nM) and Mx1 (IC50=20 nM); effectively blocks TLR3-dependent IRF3 nuclear translocation in cells with IC50<100 nM.
[Related Catalog]:
[Target]
IKKε:59 nM (IC50)
TBK1:13 nM (IC50)
[In Vitro]
TBK1/IKKε-IN-4 (Compound II; 96 hours; A549 andHCC44 cells) treatmentdisplays selective toxicity in TBK1-dependent cancer cell lines (IC50 of ~ 4.2 µM for H441 cells and IC50 of ~0.4 µM for A549 cells)[1]. TBK1/IKKε-IN-4 (Compound II; 0-2 µM; 30 minutes; HCC44 cells) treatment inhibits the AKT activity[1]. TBK1/IKKε-IN-4 (Compound II) inhibits LPS-induced expression of IFNβ (IC50 =62 nM), and the IFNβ target genes IP10 (IC50 =78 nM) and Mx1 (IC50=20 nM). TBK1/IKKε-IN-4 effectively blocksTLR3-dependent IRF3 nuclear translocation in cells with an IC50 under 100 nM, but does not impair TNFR1-dependent p65 NFκB nuclear translocation with doses as high as 20 µM[1]. Western Blot Analysis[1] Cell Line: HCC44 cells Concentration: 0 µM, 0.5 µM, 1 µM, 2 µM Incubation Time: 30 minutes Result: Was sufficient to blunt baseline AKT activity.
[References]
Chemical & Physical Properties
[ Density]:
1.3±0.1 g/cm3
[ Boiling Point ]:
668.1±65.0 °C at 760 mmHg
[ Molecular Formula ]:
C28H35N7O4
[ Molecular Weight ]:
533.622
[ Flash Point ]:
357.9±34.3 °C
[ Exact Mass ]:
533.275024
[ LogP ]:
2.85
[ Vapour Pressure ]:
0.0±2.0 mmHg at 25°C
[ Index of Refraction ]:
1.633