p-nitrophenyl alpha-l-arabinopyranoside

Names

[ Name ]:
p-nitrophenyl alpha-l-arabinopyranoside

[Synonym ]:
(2S,3R,4S,5S)-2-(4-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol

Biological Activity

[Description]:

p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection[1][3].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

p-Nitrophenyl α-L-arabinopyranoside 与 rBgaA 的亲和度 Km 值为 6.06 mM[1]。 p-Nitrophenyl α-L-arabinopyranoside 对 xylosidase–arabinosidase (xarB) 基因具有高活性[2]。 p-Nitrophenyl α-L-arabinopyranoside 在酶活性检测中的应用[3] (1) 200 µL 2 mM p-Nitrophenyl α-L-arabinopyranoside、100 µL 酶、300 µL 50 mM 磷酸盐缓冲液 (pH 7.0)，在 37℃ 下共孵育 0.5、1 和 5 h。 (2) 加入 400 µL 0.5 M 氢氧化钠，终止反应。 (3) 在 405 nm 处测量混合物的吸光度。注：在人参皂苷存在的情况下，用丁醇萃取终止反应。

[References]

[1]. Kosugi A, et al. Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation. J Bacteriol. 2002 Dec;184(24):6859-65.

[2]. Mai V, et al. Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus. Gene. 2000 Apr 18;247(1-2):137-43.

[3]. Shin H Y, et al. Purification and Characterization ofα-l-Arabinopyranosidase andα-l-Arabinofuranosidase from Bifidobacterium breve K-110, a Human Intestinal Anaerobic Bacterium Metabolizing Ginsenoside Rb2 and Rc[J]. Applied and environmental microbiology, 2003, 69(12): 7116-7123.

Chemical & Physical Properties

[ Density]:
1.597g/cm3

[ Boiling Point ]:
523.2ºC at 760mmHg

[ Melting Point ]:
205ºC

[ Molecular Formula ]:
C₁₁H₁₃NO₇

[ Molecular Weight ]:
271.22300

[ Flash Point ]:
270.2ºC

[ Exact Mass ]:
271.06900

[ PSA ]:
124.97000

[ Vapour Pressure ]:
8.94E-12mmHg at 25°C

[ Index of Refraction ]:
1.654

[ Storage condition ]:
−20°C

MSDS

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Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Synthetic Route

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Precursor & DownStream

Precursor

DownStream

Related Compounds

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