licopyranocoumarin

Licopyranocoumarin is an isoflavonoid that shows CYP3A4 inhibitory activity with an IC50 of 32 μM. Licopyranocoumarin has potent neuroprotective activities[1].

Signaling Pathways >> Metabolic Enzyme/Protease >> Cytochrome P450

Research Areas >> Neurological Disease

CYP3A4:32 μM (IC50)

Licopyranocoumarin（0.3-3 μM；48 小时）显着阻断 MPP+ 诱导的神经元 PC12D 细胞死亡和线粒体膜电位消失，这是由 JNK[1] 介导的。 Licopyranocoumarin（0.3-3 μM；36 小时）通过抑制活性氧 (ROS) 的生成来抑制 MPP+ 诱导的 JNK 活化，从而抑制 MPP+ 诱导的神经元 PC12D 细胞死亡[1]。 Cell Viability Assay[1] Cell Line: PC12D cell Concentration: 0.3 μM, 1 μM, 3 μM Incubation Time: 48 hours Result: Inhibited MPP+-induced cell death. Western Blot Analysis[1] Cell Line: PC12D cell Concentration: 0.3 μM, 1 μM, 3 μM Incubation Time: 36 hours Result: Attenuated JNK activity induced by MPP+.

[1]. Takahiro Fujimaki, et al. Identification of licopyranocoumarin and glycyrurol from herbal medicines as neuroprotective compounds for Parkinson's disease. PLoS One. 2014 Jun 24;9(6):e100395.  

[2]. Sachiko Tsukamoto, et al. CYP3A4 inhibitors isolated from Licorice. Biol Pharm Bull. 2005 Oct;28(10):2000-2.