10.1038/nmeth.4661

2018-04-09

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves minimal uncertainty in 3D on any microscope and is compatible with any PSF engineering approach. We used this method to...

10.1038/nmeth.4662

2018-04-09

To understand stem cell differentiation along multiple lineages, it is necessary to resolve heterogeneous cellular states and the ancestral relationships between them. We developed a robotic miniaturized CEL-Seq2 implementation to carry out deep single-cell R...

10.1038/nmeth.4640

2018-04-03

How to pull the blanket off dormant cancer cells How to pull the blanket off dormant cancer cells, Published online: 03 April 2018; doi:10.1038/nmeth.4640When asleep, cancer cells can evade chemo. When they wake up, they can cause cancer recurrence. By deciph...

10.1038/nmeth.4642

2018-04-03

Points of Significance: Statistics versus machine learning Points of Significance: Statistics versus machine learning, Published online: 03 April 2018; doi:10.1038/nmeth.4642Statistics draws population inferences from a sample, and machine learning finds gene...

10.1038/nmeth.4646

2018-04-03

Genomics: Single-cell hat trick Genomics: Single-cell hat trick, Published online: 03 April 2018; doi:10.1038/nmeth.4646Genomics: Single-cell hat trick

10.1038/nmeth.4650

2018-04-03

Genomics: Off-targets in epigenome editing Genomics: Off-targets in epigenome editing, Published online: 03 April 2018; doi:10.1038/nmeth.4650Genomics: Off-targets in epigenome editing

10.1038/nmeth.4648

2018-04-03

Stem cells: From somatic cells to naive pluripotent cells Stem cells: From somatic cells to naive pluripotent cells, Published online: 03 April 2018; doi:10.1038/nmeth.4648Stem cells: From somatic cells to naive pluripotent cells

10.1038/nmeth.4652

2018-04-03

Genomics: Genome editing with ease Genomics: Genome editing with ease, Published online: 03 April 2018; doi:10.1038/nmeth.4652Genomics: Genome editing with ease

10.1038/nmeth.4654

2018-04-03

Neuroscience: Minimally invasive optogenetics Neuroscience: Minimally invasive optogenetics, Published online: 03 April 2018; doi:10.1038/nmeth.4654Upconversion nanoparticles can serve as intermediaries to illuminate optogenetic tools in the mouse brain.

10.1038/nmeth.4656

2018-04-03

Cell biology: Live-streaming the cytoplasm Cell biology: Live-streaming the cytoplasm, Published online: 03 April 2018; doi:10.1038/nmeth.4656A new approach uses beams of light to direct cytoplasmic flows.